Hepatitis A Surface Antigen Antibody (BGN/B5B3 (B5-B3)) [Alexa Fluor® 750]

Novus Biologicals | Catalog # NB100-62197AF750

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

ELISA, Radioimmunoassay

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # BGN/B5B3 (B5-B3)
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Product Specifications

Immunogen

Lysate from foetal kidney cells of rhesus monkey infected with extract from patient with active viral Hepatitis A

Reactivity Notes

Viral. Not yet tested in other species.

Specificity

NB100-62197 is specific for Hepatitis A surface antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Radioimmunoassay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Hepatitis A Surface Antigen

Hepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase. Though he clinical picture of viral (A) hepatitis is extremely variable - ranging from asymptotic infection without jaundice to a fulminating disease and death in a few days, Hepatitis A is usually a mild illness and does not lead to chronic or persistent hepatitis. HAV is transmitted by the orofecal route and is still endemic throughout much of the world, including the western world. HAV exists worldwide as a single serotype with a small degree of antigenetic variation. Present evidence suggests that immunity acquired naturally (or from an inactivated hepatitis A vaccine) will protect against all human HAV strains.

Additional Hepatitis A Surface Antigen Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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