Human Hepatitis D Virus Antigen ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60046

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human hepatitis D virus(HDV) antigen. No significant cross-reactivity or interference between human hepatitis D virus(HDV) antigen and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Kit Contents for Human Hepatitis D Virus Antigen ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antigen Lysate Buffer
  • Coated assay plate
  • HRP-conjugate
  • Instruction manual
  • Negative Control
  • Positive Control
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hepatitis D Virus Antigen

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase (HRP). Any HDV antigen present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human hepatitis D virus(HDV) antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Hepatitis D Virus Antigen Products

Product Documents for Human Hepatitis D Virus Antigen ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Hepatitis D Virus Antigen ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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