Q: Good morning, we have to work with short-HepG2 Whole Cell Lysate 1.0mg to do adsorb on nanoparticles. We are chemical-physical. I would like to know if the sample handling under the hood (not flow alminare) will have a biohazard and what precautions should be taken.

A: NBP1-42569 is a whole cell lysate with the cells in a RIPA buffer. These cells are lysed and not longer viable. They are not considered a biohazard and no special precautions need to be taken.

Q: How was this lysate prepared? Please advise as I am trying to use this material as a control lysate for a kit I purchased.

A: This lysate was prepared using standard RIPA buffer with 0.1% Triton X-100, and 1% SDS and sodium deoxycholate. We used mechanical separation to remove the cells from the plate and also use a cocktail of protease and phosphatase inhibitors in the lysate buffer.

HepG2 Whole Cell Lysate

Novus Biologicals | Catalog # NBP1-42569

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Citations (1)

Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Immunoprecipitation, SDS-PAGE, Western Blot

Product Specifications

Application Notes

This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Type

Cell

Tissue

Liver

Protein State

Native

Subcellular Fraction

Whole

Scientific Data Images for HepG2 Whole Cell Lysate

Western Blot: HepG2 Whole Cell Lysate [NBP1-42569]

Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].

SDS-PAGE: HepG2 Whole Cell Lysate [NBP1-42569]

SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.

Formulation, Preparation, and Storage

Formulation

Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted. BME has not been added.

Concentration

2.5 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: HepG2

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Alternate Names

CoCl2

Additional HepG2 Products

Product Documents for HepG2 Whole Cell Lysate

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HepG2 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for HepG2 Whole Cell Lysate

Customer Reviews for HepG2 Whole Cell Lysate (1)

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Name: Anonymous

Application: Western Blot

Sample Tested: Human

Species: Human

Verified Customer | Posted 03/13/2014

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