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HIF1A Peptide Substrate, Biotin

Catalog #: S-340 Datasheet / COA / SDS

Discontinued Product

S-340 has been discontinued.
View all HIF-1 alpha/HIF1A products.
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Summary Product Datasheets Background

HIF1A Peptide Substrate, Biotin Summary

Specifications

Source
Synthetic
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storeat -20°C or -80°C.  Avoid multiplefreeze/thaw cycles.
Applications
Bioactivity
Species
Human

Product Datasheets

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Product Datasheet
COA
SDS

Background: HIF-1 alpha/HIF1A

This biotinylated peptide includes a sequence from human Hypoxia-Inducible Factor 1-alpha (HIF-1 alpha ) including a 4-hydroxyline proline residue corresponding to proline 564 in UniProt Q16665.  The peptide can be ubiquitinated by a VHL/Elongin B/Elongin C/CUL2/RBX complex (catalog number E3-655) in vitro using wild-type ubiquitin or lysine-less ubiquitin ("Ub-NOK").  A matched negative control peptide (catalog S-345) with an unmodified proline residue is available; depending on experimental conditions the negative control peptide is ubiquitinated at ≤ 5% of the level of the peptide containing 4-hydroxyproline.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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