Home » His Tag » His Tag Alexa Fluor® 350-conjugated Antibody
His Tag Alexa Fluor® 350-conjugated Antibody
Recombinant Monoclonal Antibody.
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus.
Monoclonal Mouse IgG1 Clone # AD1.1.10R
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 350
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with His-tagged proteins
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
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