His Tag Antibody

Recombinant Monoclonal Antibody
  (2 citations)
(6 Reviews)
    
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Product Details
Citations (2)
FAQs
Supplemental Products
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  • Specificity
    Detects proteins containing accessible consecutive histidine regions.  The antibody detects His tags located at the amino- or carboxyl- terminus.
  • Source
    Recombinant Monoclonal Mouse IgG1 Clone # AD1.1.10R
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    His-tagged peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoaffinity Purification
    Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Recombinant Monoclonal Antibody
(Catalog # MAB050R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins were stained with Mouse Anti-His Tag Recombinant Monoclonal Antibody (Catalog # MAB050R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
  • Long Name:
    Histidine Tag
  • Alternate Names:
    6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Lipid bilayer simulations of CXCR4 with inverse agonists and weak partial agonists.
    Authors: Trent J, Wang Z, Murray J, Shao W, Tamamura H, Fujii N, Peiper S
    J Biol Chem, 0;278(47):47136-44.
    Application: WB
  2. Paradoxical roles of ATF6α and ATF6β in modulating disease severity caused by mutations in collagen X.
    Authors: Forouhan M, Mori K, Boot-Handford R
    Matrix Biol, 0;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 151  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 33  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 12
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002 1  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 5 (Based on 6 reviews)

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Read what people are saying who have used His Tag Antibody.

We have 6 reviews tested in 2 species: Human, Mouse.
We have 6 review tested in 1 application: Flow Cytometry.

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Excellent
(6)
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Flow
(6)
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Human
(1)
Mouse
(5)
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Images Ratings Applications Species Reviewed By Date Details
Excellent
 Flow Mouse Anonymous 04/12/2018
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Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
Excellent
 Flow Mouse Anonymous 03/30/2018
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Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse
Excellent
 Flow Mouse Anonymous 03/15/2018
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Summary

Application Flow Cytometry
Sample Tested B16-F10 mouse melanoma cell line
Species Mouse
Excellent
 Flow Mouse Anonymous 03/11/2018
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Summary

Application Flow Cytometry
Sample Tested 786-O human renal cell adenocarcinoma cell line
Species Mouse
Excellent
 Flow Human Anonymous 12/18/2017
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Summary

Application Flow Cytometry
Sample Tested MCF 10A human breast epithelial cell line
Species Human
Flow Cytometry His Tag Antibody MAB050R Array MAB050R
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Excellent
 Flow Mouse Anonymous 11/27/2017
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Image Details
Flow Cytometry His Tag Antibody MAB050R
Flow Cytometry: His Tag Antibody [MAB050R].

Summary

Application Flow Cytometry
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse

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