Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10R
View all His Tag Primary Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions.  The antibody detects His tags located at the amino- or carboxyl- terminus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag PE‑conjugated Antibody

Detection of protein binding with Mouse anti-His Tag PE in HEK293 cells by Flow Cytometry

In a functional flow cytometry test, recombinant SARS-CoV2 S1 RBD His-tagged protein (50 ng/mL, 10500-CV) binds to HEK293 cells transfected with Human ACE-2 and eGFP. (A) Protein binding was detected with Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) or Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) alone. View our protocol for View our protocol for Staining Membrane-associated Proteins.

Applications for His Tag PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 cells transfected with Human ACE-2 and eGFP.  

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for His Tag PE‑conjugated Antibody

For research use only

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