HIST3H3 Antibody (4E9B11) - BSA Free

Novus Biologicals | Catalog # NBP2-52482

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E9B11

Format

BSA Free
View all HIST3H3 Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide of human HIST3H3 (AA: ARTKQTAR(AcK)STG-C).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIST3H3 Antibody (4E9B11) - BSA Free

Western Blot: HIST3H3 Antibody (4E9B11)BSA Free [NBP2-52482]

Western Blot: HIST3H3 Antibody (4E9B11)BSA Free [NBP2-52482]

Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.
Flow Cytometry: HIST3H3 Antibody (4E9B11) - BSA Free [NBP2-52482]

Flow Cytometry: HIST3H3 Antibody (4E9B11) - BSA Free [NBP2-52482]

Flow Cytometry: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).
ELISA: HIST3H3 Antibody (4E9B11) - BSA Free [NBP2-52482]

ELISA: HIST3H3 Antibody (4E9B11) - BSA Free [NBP2-52482]

ELISA: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for HIST3H3 Antibody (4E9B11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIST3H3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The HIST3H3 gene is intronless and encodes a 136 amino acid long, 15 kDA histone H3.1t protein that lacks polyA tails and obtain a palindromic termination element. The HIST3H3 gene is located separately from the other H3 genes on the cluster on chromosome 6p22-p21.3. HIST3H3 participates in PKA signaling, development notch signaling pathway, histone modification, and interferon pathway through interactions with genes HIST1H4B, HIST4H4, HIST1H4A, HIST1H4C, and HIST1H4D. HIST3H3 has been researched regarding its role in immunodeficiency, leukemia, prostatitis, huntington's disease, lupus, multiple sclerosis, laryngeal cancer, and lymphomas.

Long Name

Histone Cluster 3 H3

Alternate Names

H3.4, H3/G, H3/T, H3FT, H3t, Histone 3, H3

Gene Symbol

H3-4

Additional HIST3H3 Products

Product Documents for HIST3H3 Antibody (4E9B11) - BSA Free

Certificate of Analysis

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Product Specific Notices for HIST3H3 Antibody (4E9B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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