Histamine Antibody
Novus Biologicals | Catalog # NBP2-45266
![Immunocytochemistry: Histamine Antibody [NBP2-45266]](https://resources.rndsystems.com/images/products/Histamine-Antibody-Immunocytochemistry-NBP2-45266-img0002.jpg "Immunocytochemistry: Histamine Antibody [NBP2-45266]")
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Key Product Details
Species Reactivity
Validated:
Human, All Species
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Dot Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Histamine conjugated to succinylated KLH.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 30758643).
Specificity
The product specifically stains histamine-containing cells in paraformaldehyde/carbodiimide-fixed or paraformaldehyde-fixed, paraffin-embedded sections of rat stomach (endocrine cells and mast cells).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histamine Antibody
Immunocytochemistry: Histamine Antibody [NBP2-45266]
Immunocytochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:100 Anti-Histamine antibody produced in Rabbit, followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.![Immunohistochemistry: Histamine Antibody [NBP2-45266]](https://resources.rndsystems.com/images/products/Histamine-Antibody-Immunohistochemistry-NBP2-45266-img0001.jpg "Immunohistochemistry: Histamine Antibody [NBP2-45266]")
Immunohistochemistry: Histamine Antibody [NBP2-45266]
Immunohistochemistry: Histamine Antibody [NBP2-45266] - Formalin fixed, paraffin-embedded Rat stomach tissue sections were stained with 1:200 Anti-Histamine antibody produced in Rabbit (Cat. No. H7403), followed by 25 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 25 ug/mL ExtrAvidin-Peroxidase.Applications for Histamine Antibody
Application
Recommended Usage
Dot Blot
1:14000
Immunohistochemistry-Paraffin
1:100 - 1:200
Application Notes
Dot Blot: 1:14,000 using histamine-BSA.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histamine
Alternate Names
2 (4 imidazolyl)ethylamine, 4 (2 aminoethyl) 1 3 diazole
Additional Histamine Products
Product Documents for Histamine Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histamine Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Histamine Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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