Histamine H3R Antibody (27H8) - BSA Free

Novus Biologicals | Catalog # NBP3-26219

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 27H8 expressed in HEK293

Format

BSA Free
View all Histamine H3 R Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Histamine H3R [UniProt Q9Y5N1]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histamine H3R Antibody (27H8) - BSA Free

Histamine H3R Antibody (27H8)

Flow Cytometry: Histamine H3R Antibody (27H8) [NBP3-26219] -

Flow Cytometry: Histamine H3R Antibody (27H8) [NBP3-26219] - Overlay Peak curve showing SH-SY5Y cells stained with Histamine H3R Antibody (27H8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Histamine H3R Antibody (27H8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Histamine H3 R

H3 Histamine Receptor regulates the release of the neurotransmitters norepinephrine and somatostatin. In histamine-containing neurons of the brain, the H3 receptor acts as a presynaptic autoreceptor, while in nonhistamine-containing neurons in both the central and peripheral nervous systems, it acts as a presynaptic heteroreceptor. Multiple isoforms of H3 receptors are produced by alternative splicing. Histamine H3 receptor has been reported primarily in brain. ESTs have been isolated from brain (normal and cancer), breast cancer, colon cancer, and retinoblastoma libraries.

Long Name

Histamine H3 Receptor

Alternate Names

GPCR97, HH3R, Histamine H3R, HRH3

Gene Symbol

HRH3

Additional Histamine H3 R Products

Product Documents for Histamine H3R Antibody (27H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histamine H3R Antibody (27H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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