Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-62793

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone H2A Primary Antibodies »

Product Specifications

Immunogen

Region of histone H2A containing the ubiquitinylated lysine 119 (H2AK119ub)

Modification

ubiquitylated Lys119

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]

Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]

Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Analysis was performed on whole cell extracts from HeLa cells (25 ug, lane 1), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub.
Chromatin Immunoprecipitation (ChIP): Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Assays were performed using human HeLa cells, the antibody against H2AK119ub and optimized PCR primer pairs for qPCR. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]

ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]

ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the Diagenode antibody against H2AK119ub. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:23,000.
Dot Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To test the cross reactivity of the antibody against H2AK119ub, a Dot Blot analysis was performed with peptides containing other histone ubiquitinylations and unmodified sequences. This Figure shows a high specificity of the antibody for the modification of interest.

Applications for Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2 - 5 ug

Dot Blot

1:20000

ELISA

1:200

Western Blot

1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2A

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Long Name

H2A Histone Family

Alternate Names

H2A1B, HIST1H2AB

Gene Symbol

H2AC20

Additional Histone H2A Products

Product Documents for Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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