Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Histone H2AX [p Ser139] Antibody (BLR053F)
Novus Biologicals | Catalog # NBP3-14678
Recombinant Monoclonal Antibody
![Western Blot: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Western-Blot-NBP3-14678-img0002.jpg "Western Blot: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR053F
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Product Specifications
Immunogen
surrounding Serine 140
Modification
p Ser139
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for Histone H2AX [p Ser139] Antibody (BLR053F)
Western Blot: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Western Blot: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds.![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Immunocytochemistry-Immunofluorescence-NBP3-14678-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human HeLa cells treated with etoposide. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0007.jpg "Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human breast carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:100. Secondary: DyLight(R) 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.![Flow Cytometry: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Flow-Cytometry-NBP3-14678-img0008.jpg "Flow Cytometry: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Flow Cytometry: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Flow Cytometry: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Rabbit Histone H2AX antibody (BLR053F) or isotype control (unshaded). Secondary: DyLight(R) 488-conjugated goat anti-rabbit IgG.![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0003.jpg "Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of human basal cell carcinoma. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-BLR053F-Immunohistochemistry-Paraffin-NBP3-14678-img0005.jpg "Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Section of mouse CT26 tumor. Antibody: Rabbit Histone H2AX antibody (BLR053F) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate:DAB.![Immunoprecipitation: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]](https://resources.rndsystems.com/images/products/gamma-H2AX-Antibody-Immunoprecipitation-NBP3-14678-img0001.jpg "Immunoprecipitation: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]")
Immunoprecipitation: Histone H2AX [p Ser139] Antibody (BLR053F) [NBP3-14678]
Immunoprecipitation: Histone H2AX Antibody (BLR053F) [NBP3-14678] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-gamma-H2AX recombinant monoclonal antibody used for IP at 20 ul per reaction. gamma-H2AX was also immunoprecipitated by rabbit anti-gamma-H2AX antibody. For blotting immunoprecipitated gamma-H2AX, was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds. Lower panel: Rabbit anti-H2AXApplications for Histone H2AX [p Ser139] Antibody (BLR053F)
Application
Recommended Usage
Flow Cytometry
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
20 ul/1mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: Histone H2AX
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX [p Ser139] Antibody (BLR053F)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2AX [p Ser139] Antibody (BLR053F)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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