Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43837

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5256

Format

Azide and BSA Free
View all Histone H2AY Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O75367, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free

Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Staining of human kidney shows strong nuclear positivity in renal glomeruli and renal tubules cells.
Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in lamina propria cells.
Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Staining of human cervix shows strong nuclear positivity in squamous epithelial cells and in connective tissue cells.
Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-43837]

Staining of human prostate shows strong nuclear immunoreactivity in glandular and smooth muscle cells.

Applications for Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone H2AY

macroH2A.1 (H2AFY) is 372 amino acids long, weighs approximately 40 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. macroH2a.1 has two shorter isoforms meeasuring 371 and 369 amino acids long, both weighing approximately 39 kDa. Current studies are being done on several diseases and disorders including medulloblastoma, lupus erythematosus, Huntington's disease, immunodeficiency, melanoma, and malaria. macroH2A.1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the chromatin regulation/ acetylation, systemic lupus erythematosus, and ATF-2 transcription factor pathways.

Long Name

H2A Histone Family, Member Y

Alternate Names

H2AF12M, H2AFJ, H2AFY, Histone MacroH2A1, Medulloblastoma Antigen MU-MB-50.205

Gene Symbol

MACROH2A1

Additional Histone H2AY Products

Product Documents for Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H2AY/macroH2A.1 Antibody (CL5256) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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