Histone H3 [p Ser10, ac Lys9] Antibody - BSA Free

Novus Biologicals | Catalog # NB21-1095

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all Histone H3 Primary Antibodies »

Product Specifications

Immunogen

Anti-Histone H3 [ac Lys9/phospho Ser10] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a acetylation surrounding Lysine 9 and a phosphorylation surrounding serine 10 of human Histone H3.

Modification

p Ser10, ac Lys9

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Histone H3 [p Ser10, ac Lys9] Antibody - BSA Free

Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] Antibody

Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] Antibody

Western Blot analysis of Histone H3 [ac Lys9/phospho Ser10] in NIH/3T3 cell lysates using NB21-1095.
Histone H3 [p Ser10, ac Lys9] Antibody Histone H3 [p Ser10, ac Lys9] Antibody Dot Blot Analysis

Dot Blot analysis of Histone H3 [p Ser10, ac Lys9] Antibody

Dot Blot analysis of Histone H3 [p Ser10, ac Lys9] Antibody in the peptides listed in 1.0 and 0.5 picomoles of peptide.
Histone H3 [p Ser10, ac Lys9] Antibody Immunocytochemistry/Immunofluorescence analysis of Histone H3 [ac Lys9/phospho Ser10] Antibody

Immunocytochemistry/Immunofluorescence analysis of Histone H3 [ac Lys9/phospho Ser10] Antibody

Histone H3 K9-Ac/pS10 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Applications for Histone H3 [p Ser10, ac Lys9] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 30% Glycerol

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

0.25 mg/ml

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Alternate Names

H3F3A

Gene Symbol

H3C14

Additional Histone H3 Products

Product Documents for Histone H3 [p Ser10, ac Lys9] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone H3 [p Ser10, ac Lys9] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Interferons
Notch Pathway

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Protocols

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