HIV-1 Gag p24 Quantikine ELISA Kit

R&D Systems | Catalog # DHP240B

Validated for Cell Culture Supernates Only
R&D Systems
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL)

Sensitivity

3.35 pg/mL

Assay Range

7.81-500 pg/mL (Cell Culture Supernates)
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Product Summary for HIV-1 Gag p24 Quantikine ELISA Kit

The Quantikine™ HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Viral

Specificity

This assay recognizes HIV-1 Gag p24.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.7 135 280 43.0 132 285
Standard Deviation 1.41 4.86 8.10 1.93 4.61 11.2
CV% 3.1 3.6 2.9 4.5 3.5 3.9

Recovery for HIV-1 Gag p24 Quantikine ELISA Kit

The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 93-105

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data Images for HIV-1 Gag p24 Quantikine ELISA Kit

HIV-1 Gag p24 Quantikine ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

Additional HIV-1 Gag p24 Products

Product Documents for HIV-1 Gag p24 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for HIV-1 Gag p24 Quantikine ELISA Kit

For research use only

Related Research Areas

Infectious Diseases

Citations for HIV-1 Gag p24 Quantikine ELISA Kit

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  • HIV-1 Gag p24 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: 293T human embryonic kidney cell line
    Verified Customer | Posted 12/21/2020
    I used this kit to quantify HIV-1 Gag VLPs in cell culture supernatants. I adsorb the VLPs on polymer membranes and desorb them by vortexing/ centrifugation. I use HIV-1 Gag p24 ELISA kit to determine how polymer surface chemistry can be manipulated to control reversible adsorption and desorption of VLPs.
    HIV-1 Gag p24 Quantikine ELISA Kit DHP240B

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