HIV-1 gp120 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06630

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HIV-1 gp120 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant HIV-1 gp120 (Ala29-Glu502; Accession#:AFM77980.1).

Reactivity Notes

Human Immunodeficiency Virus type 1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HIV-1 gp120 Antibody - Azide and BSA Free

HIV-1 gp120 Antibody

Western Blot: HIV-1 gp120 Antibody [NBP3-06630] -

Western Blot: HIV-1 gp120 Antibody [NBP3-06630] - NBP3-06630 at 1:1000 dilution. Sample: HIV-1 gp120 Protein (group M, subtype CRF07_BC) Recombinant Protein. Lane A: 50ng Lane B: 10ng Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.

Applications for HIV-1 gp120 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:5000-1:10000
Application Notes
Western Blot: Using a DAB detection system, the detection limit for HIV-1 gp120 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIV-1 gp120

HIV-1 gp120 is a surface glycoprotein that enables viral attachment and entry into host cells. It is also involved in neurotoxicity and has been implicated in HIV-associated neurocognitive disorders. HIV-1 gp120 also induces apoptosis in neuronal cells by signaling mitochondrial-death proteins which may upregulate the death receptor, Fas. Because HIV gp120 is necessary for HIV-1 to enter CD4 cells, gp120 is one of the main targets of HIV vaccine research.

Alternate Names

Envelope surface glycoprotein gp120, gp120, gp120 glycoprotein, Human immunodeficiency virus 1, SU, Surface protein

Gene Symbol

env

Additional HIV-1 gp120 Products

Product Documents for HIV-1 gp120 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HIV-1 gp120 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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