HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-43553

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BB7.2

Format

BSA Free
View all HLA A2 Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA A and B7 antigens. (Uniprot# P04439)

Specificity

This antibody binds the carboxy terminus of the alpha2-helix of class I MHC molecule and a turn on one of the underlying beta-strands.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

Western Blot: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] -

Western Blot: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] - Daudi cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with NBP3-43553 at 0.3ug/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

Immunocytochemistry /Immunofluorescence: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] -

Immunocytochemistry /Immunofluorescence: HLA A2 Antibody (BB7.2) - Chimeric - BSA Free [NBP3-43553] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with NBP3-43553 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43553, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Applications for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA A2

Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Additional HLA A2 Products

Product Documents for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HLA A2 Antibody (BB7.2) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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