HLA Class I Antibody (MEM-147) [DyLight 405]

Novus Biologicals | Catalog # NB500-411V

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-147
View all HLA Class I Primary Antibodies »

Product Specifications

Immunogen

PHA-activated peripheral blood lymphocytes

Specificity

This antibody (clone MEM-147) reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for HLA Class I Antibody (MEM-147) [DyLight 405]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HLA Class I

HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.

Long Name

Class I Human Leukocyte Antigen

Alternate Names

HLA-A, HLA-ABC

Entrez Gene IDs

3107 (Human); 3106 (Human)

Gene Symbol

HLA-C

Additional HLA Class I Products

Product Documents for HLA Class I Antibody (MEM-147) [DyLight 405]

Certificate of Analysis

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Product Specific Notices for HLA Class I Antibody (MEM-147) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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