HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20840

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rHLA-DRB/7198

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant full-length human HLA DRB1 protein

Localization

Cell surface.

Specificity

This monoclonal antibody reacts with a 28kDa chain of HLA DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: Raji, Ramos, Daudi or HuT78 cells. Human lymphoid tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

HLA DRB1 Antibody (rHLA-DRB/7198)

Formalin-fixed, paraffin-embedded human tonsil stained with HLA DRB1 antibody (rHLA-DRB/7198). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HLA-DRB1

HLA DRB1 can be used for HLA typing.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Gene Symbol

HLA-DRB1

Additional HLA-DRB1 Products

Product Documents for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HLA DRB1 Antibody (rHLA-DRB/7198) - Azide and BSA Free

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  • Q: We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?

    A:

    While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

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