HNF-3 beta/FoxA2 Antibody

Novus Biologicals | Catalog # NB100-1263

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HNF-3 beta/FoxA2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GVYSRPIMNSS corresponding to C-Terminus according to NP_710141.1.

Localization

Nuclear

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HNF-3 beta/FoxA2 Antibody

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263]

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Applications for HNF-3 beta/FoxA2 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 8 ug/ml

Peptide ELISA

Detection limit 1:4000

Western Blot

1-3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human); 15376 (Mouse); 25099 (Rat)

Gene Symbol

FOXA2

UniProt

NP_068556 (Human)

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody

Certificate of Analysis

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Product Specific Notices for HNF-3 beta/FoxA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HNF-3 beta/FoxA2 Antibody

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Protocols

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