hnRNP U Antibody

Novus Biologicals | Catalog # NB100-2134

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Immunoprecipitation, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all hnRNP U Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human Heterogeneous Nuclear Ribonucleoprotein U using the numbering given in entry NP_114032.1 (GeneID 3192).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP U Antibody

Western Blot: hnRNP U Antibody [NB100-2134]

Western Blot: hnRNP U Antibody [NB100-2134]

Western Blot: hnRNP U Antibody [NB100-2134] - Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 10seconds.
Immunohistochemistry-Paraffin: hnRNP U Antibody [NB100-2134]

Immunohistochemistry-Paraffin: hnRNP U Antibody [NB100-2134]

Immunohistochemistry-Paraffin: hnRNP U Antibody [NB100-2134] - Human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-U used at a dilution of 1:200 (1 ug/mL). Detection: DAB
Western Blot: hnRNP U Antibody [NB100-2134]

Western Blot: hnRNP U Antibody [NB100-2134]

Western Blot: hnRNP U Antibody [NB100-2134] - Detection of human hnRNP-U by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-hnRNP-U antibody NB100-2134 used for WB at 1 ug/ml. hnRNP-U was immunoprecipitated using a rabbit anti-hnRNP-U antibody at 3 ug/mg lysate. Detection: Chemiluminescence with exposure time of 30 seconds.
Simple Western: hnRNP U Antibody [NB100-2134]

Simple Western: hnRNP U Antibody [NB100-2134]

Simple Western: hnRNP U Antibody [NB100-2134] - Electropherogram from Simple Western (WES) of HNRNPU (NB100-2134). Antibody at 1:100 on protein sample from Neural Epithelial Stem Cells. Simple Western image submitted by a verified customer review.

Applications for hnRNP U Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Simple Western

1:100

Western Blot

1:2000 - 1:10000
Application Notes
Immunoprecipitation: Not Recommended, use rabbit anti-hnRNP-U antibody NB100-2135. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. This hnRNP U antibody is validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in Neural Epithelial Stem Cells, separated by Size, antibody dilution of 1:100

Reviewed Applications

Read 1 review rated 4 using NB100-2134 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: hnRNP U

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Alternate Names

heterogeneous nuclear ribonucleoprotein U, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), hnRNP U, HNRPU, p120, p120 nuclear protein, pp120, SAFA, SAF-AhnRNPU, Scaffold attachment factor A, U21.1

Entrez Gene IDs

3192 (Human); 51810 (Mouse)

Gene Symbol

HNRNPU

UniProt

Q00839 (Human)

Additional hnRNP U Products

Product Documents for hnRNP U Antibody

Certificate of Analysis

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Product Specific Notices for hnRNP U Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hnRNP U Antibody

Customer Reviews for hnRNP U Antibody (1)

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Customer Images

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  • hnRNP U Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Human fibroblast and neural stem cells
    Species: Human
    Verified Customer | Posted 11/28/2019
    Elecropherogram from Simple Western (WES) of HNRNPU (NB100-2134) 1:100 on protein sample from Neural Epithelial Stem Cells
    Dilution 1:100
    hnRNP U Antibody NB100-2134

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Protocols

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