Hormone-sensitive Lipase/HSL Antibody

Novus Biologicals | Catalog # NB110-37253

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Hormone-sensitive Lipase/HSL Primary Antibodies »

Product Specifications

Immunogen

A fusion protein containing an internal portion of the rat HSL protein (between residues 699-908). [Swiss-Prot P15304]

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hormone-sensitive Lipase/HSL Antibody

Western Blot: Hormone-sensitive Lipase/HSL Antibody [NB110-37253]

Western Blot: Hormone-sensitive Lipase/HSL Antibody [NB110-37253]

Western Blot: HSL Antibody [NB110-37253] - Detection of HSL using NB110-37253. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample).

Applications for Hormone-sensitive Lipase/HSL Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This HSL antibody is useful for Western Blot, where a band at ~84kDa is seen representing HSL protein. A non-specific band may be seen at ~89 kDa. Bands at ~102, 113, and 127 kDa may also be seen in the testis of sexually immature rats.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS containing 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hormone-sensitive Lipase/HSL

HSL (hormone-sensitive lipase) is a key enzyme in lipid metabolism regulation in several tissues and this intracellular neutral lipase hydrolyzes triacylglycerols (TG), diacylglycerols (DG), and monoacylglycerols, as well as cholesterol and retinyl esters. While ATGL selectively performs the first step in TG hydrolysis, HSL is the most active enzyme against DG, which gets hydrolyzed faster than TG. HSL has been shown to express in several non-adipose tissues, including ovary, adrenal gland, heart, skeletal and smooth muscle, placenta, and pancreatic beta-cells, as well as testes. Under basal conditions, HSL is exclusively found in the cytosol of adipocytes, whereas upon phosphorylation mediated activation via PKA, HSL translocates from cytosol to lipid droplet surfaces for participation in lipolysis. HSL activity is regulated primarily by serine phosphorylation, which is stimulatory at Ser-563/Ser-659 via PKA and inhibitory at Ser-565 via AMPK. Mice lacking HSL have decreased nonesterified fatty acid (NEFA) and increased cholesterol concentrations in plasma and are resistant to diet-induced obesity. HSL has emerged as a potential target for lipid disorders and obesity.

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Gene Symbol

LIPE

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Hormone-sensitive Lipase/HSL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Hormone-sensitive Lipase/HSL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bioactive Lipids and Receptors

Citations for Hormone-sensitive Lipase/HSL Antibody

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