Hornerin Antibody

Novus Biologicals | Catalog # NB100-93405

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Hornerin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QYATQHGEYDTLNK corresponding to internal region according to NP_001009931.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Hornerin Antibody

Hornerin Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal Hornerin Antibody [NB100-93405] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal Hornerin Antibody [NB100-93405] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Hornerin Antibody

Flow Cytometry: Goat Polyclonal Hornerin Antibody [NB100-93405] -

Flow Cytometry: Goat Polyclonal Hornerin Antibody [NB100-93405] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Hornerin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 50 kDa band in human skin lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 282 kDa band according to NP_001009931.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hornerin

Hornerin may play a role in cornification

Alternate Names

hornerin, intermediate filament-associated protein, S100A16, S100a18

Entrez Gene IDs

388697 (Human)

Gene Symbol

HRNR

UniProt

Q86YZ3 (Human)

Additional Hornerin Products

Product Documents for Hornerin Antibody

Certificate of Analysis

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Product Specific Notices for Hornerin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hornerin Antibody

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