HPRT Antibody (4N2Q6)

Novus Biologicals | Catalog # NBP3-16702

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4N2Q6 expressed in HEK293
View all HPRT Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 119-218 of human HPRT (NP_000185.1). GDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPRT Antibody (4N2Q6)

Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702]

Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702]

Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of extracts of various cell lines, using HPRT1 Rabbit mAb (NBP3-16702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
HPRT Antibody (4N2Q6)

Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] -

Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of lysates from wild type(WT) and HPRT knockout (KO) 293T cells, using [KO Validated] HPRT Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Applications for HPRT Antibody (4N2Q6)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Gene Symbol

HPRT1

Additional HPRT Products

Product Documents for HPRT Antibody (4N2Q6)

Certificate of Analysis

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Product Specific Notices for HPRT Antibody (4N2Q6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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