HR6B/UBE2B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20781

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rabbit (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (98%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HR6B/UBE2B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HR6B/UBE2B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Cell membrane (By similarity); Nucleus (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HR6B/UBE2B Antibody - BSA Free

Western Blot: HR6B/UBE2B Antibody [NBP2-20781]

Western Blot: HR6B/UBE2B Antibody [NBP2-20781]

Western Blot: HR6B/UBE2B Antibody [NBP2-20781] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HR6B/UBE2B Antibody [NBP2-20781]

Western Blot: HR6B/UBE2B Antibody [NBP2-20781]

Western Blot: HR6B/UBE2B Antibody [NBP2-20781] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Applications for HR6B/UBE2B Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HR6B/UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

UniProt

P63146 (Human)

Additional HR6B/UBE2B Products

Product Documents for HR6B/UBE2B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HR6B/UBE2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Epigenetics
p53 Pathway

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail