Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # H-Ras-03

Format

BSA Free
View all HRAS Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.

Specificity

The antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HRAS Antibody (H-Ras-03) - BSA Free

Western Blot: HRAS Antibody (H-Ras-03)BSA Free [NB110-68799]

Western Blot: HRAS Antibody (H-Ras-03)BSA Free [NB110-68799]

Western Blot: vH RAS Antibody (H-Ras-03) [NB110-68799] - Western Blotting analysis (reducing conditions) of vH RAS Antibody (H-Ras-03) in whole cell lysate using anti-human vH RAS Antibody (H-Ras-03). Lane 1: HeLa human cervix carcinoma cell line Lane 2: K567 human leukemia cell line Lane 3: RAJI human Burkitt lymphoma cell line.
HRAS Antibody (H-Ras-03) - BSA Free Western Blot: HRAS Antibody (H-Ras-03) - BSA Free [NB110-68799]

Western Blot: HRAS Antibody (H-Ras-03) - BSA Free [NB110-68799]

Analysis of human H-Ras using mouse monoclonal antibody H-Ras-03 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.

Applications for HRAS Antibody (H-Ras-03) - BSA Free

Application
Recommended Usage

Western Blot

0.5 -1 ug/ml
Application Notes
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Application note: Reducing conditions. Purity is > 95% (by SDS-PAGE).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HRAS

H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases vH RAS Antibody (H-Ras-03) affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of vH RAS Antibody (H-Ras-03) signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.

Alternate Names

C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Entrez Gene IDs

3265 (Human)

Gene Symbol

HRAS

UniProt

P01112 (Human)

Additional HRAS Products

Product Documents for HRAS Antibody (H-Ras-03) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HRAS Antibody (H-Ras-03) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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