HSF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97475

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Gerbil, Primate, Rabbit

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation, Gel Super Shift Assays

Cited:

Western Blot, ELISA, Immunoprecipitation, Gel Supershift Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSF1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HSF1.

Specificity

Recognizes nonphosphorylated HSF1 at ~60kDa and phosphorylated HSF1 at ~80kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSF1 Antibody - BSA Free

Western Blot: HSF1 Antibody [NBP1-97475]

Western Blot: HSF1 Antibody [NBP1-97475]

Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.

Applications for HSF1 Antibody - BSA Free

Application
Recommended Usage

Gel Super Shift Assays

1:1-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000 (colorimetric)
Application Notes
ELISA reactivity was reported in (PMID:12732732).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSF1

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Entrez Gene IDs

3297 (Human); 15499 (Mouse); 79245 (Rat)

Gene Symbol

HSF1

UniProt

Q00613 (Human)

Additional HSF1 Products

Product Documents for HSF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSF1 Antibody - BSA Free

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