HSF4 Antibody (2E7) - BSA Free

Novus Biologicals | Catalog # NBP2-37499

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E7

Format

BSA Free
View all HSF4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HSF4 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSF4 Antibody (2E7) - BSA Free

Western Blot: HSF4 Antibody (2E7)BSA Free [NBP2-37499]

Western Blot: HSF4 Antibody (2E7)BSA Free [NBP2-37499]

Western Blot: HSF4 Antibody (2E7) [NBP2-37499] - Western blot analysis using HSF4 mAb against human HSF4 (AA: 245-411) recombinant protein. (Expected MW is 42.9 kDa)
Flow Cytometry: HSF4 Antibody (2E7) - BSA Free [NBP2-37499]

Flow Cytometry: HSF4 Antibody (2E7) - BSA Free [NBP2-37499]

Flow Cytometry: HSF4 Antibody (2E7) [NBP2-37499] - Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
ELISA: HSF4 Antibody (2E7) - BSA Free [NBP2-37499]

ELISA: HSF4 Antibody (2E7) - BSA Free [NBP2-37499]

ELISA: HSF4 Antibody (2E7) [NBP2-37499] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for HSF4 Antibody (2E7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSF4

HSF4 is a gene which codes for a DNA-binding protein that binds to heat shock promoter elements and is expressed in the heart, skeletal muscle, eyes and brain, with an isoform with a length of 492 amino acids and a weight of approximately 53 kDa which activates transcription, and a shorter isoform with a length of 462 amino acids and a weight of approximately 50 kDa that represses transcription. Current studies are being done on several disorders and diseases relating to this gene including cataract, otitis media, cervical adenitis, Scheuermann's disease, Ritter's disease, and impetigo. HSF4 has also been shown to have interactions with CDK2AP2, NUDT21, C5orf54, MAPK1, and DUSP26.

Long Name

Heat Shock Transcription Factor 4

Alternate Names

AT-HSFB1, ATHSF4, CTM, HSFB1

Gene Symbol

HSF4

Additional HSF4 Products

Product Documents for HSF4 Antibody (2E7) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSF4 Antibody (2E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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