Recombinant HSP65 (mycobacterial) Protein

Novus Biologicals | Catalog # NBP3-09170

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Western Blot, ELISA, SDS-PAGE
View all HSP65 (mycobacterial) Proteins and Enzymes »

Product Specifications

Description

Recombinant protein for Bacteria HSP65 (mycobacterial)

Source:E. coli
Amino Acid Sequence: (P0A520)

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant HSP65 (mycobacterial) Protein

SDS-PAGE: Recombinant HSP65 (mycobacterial) Protein [NBP3-09170]

SDS-PAGE: Recombinant HSP65 (mycobacterial) Protein [NBP3-09170]

SDS-Page: Recombinant HSP65 (mycobacterial) Protein [NBP3-09170] - SDS-PAGE of 65kDa M. Bovis Hsp65 protein (NBP3-09170).

Formulation, Preparation, and Storage

NBP3-09170
Preparation Method This protein was expressed in E. coli and multi-step purified.
Formulation 10 mM Tris (pH 8), 105 mM NaCl, 5 mM EDTA
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HSP65 (mycobacterial)

HSP65 isolated from Mycobacterium bovis BCG, is a member of the HSP60 family of heat shock proteins. HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner. Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively. Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats.

Alternate Names

60 kDa chaperonin 2, 65 kDa antigen, Antigen A, Cell wall protein A, GroEL protein 2, groEL-2, groL2, Heat shock protein 65, hsp65, MT0456, MTV037.04, Protein Cpn60-2, Rv0440

Gene Symbol

groEL2

Additional HSP65 (mycobacterial) Products

Product Documents for Recombinant HSP65 (mycobacterial) Protein

Certificate of Analysis

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Product Specific Notices for Recombinant HSP65 (mycobacterial) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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