HSP90 Antibody (10D6) - BSA Free

Novus Biologicals | Catalog # NBP3-15144

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10D6 expressed in HEK293

Format

BSA Free
View all HSP90 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HSP90. UniProt ID (P07900/P08238).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 Antibody (10D6) - BSA Free

Western Blot: HSP90 Antibody (10D6) [NBP3-15144]

Western Blot: HSP90 Antibody (10D6) [NBP3-15144]

Western Blot: HSP90 Antibody (10D6) [NBP3-15144] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: HSP90 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa. Observed band size: 90 KDa.
Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144]

Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144]

Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144] - Overlay histogram showing Jurkat cells stained with HSP90 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144]

Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144]

Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144] - Immunoprecipitating HSP90 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of HSP90 antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: HSP90 antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).

Applications for HSP90 Antibody (10D6) - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HSP90

Hsp90 has at least 2 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (10D6) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP90 Antibody (10D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail