HT PARP in vivo Pharmacodynamic ELISA Kit II

For the quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts
Catalog # Availability Size / Price Qty
4520-096-K
Product Details
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HT PARP in vivo Pharmacodynamic ELISA Kit II Summary

Monitors in vivo PARP activity within cells based on levels of PAR.

Key Benefits

• Pre-coated 96 well capture antibody plates
• High signal to noise ratio
• Detection sensitivity of 2 pg/mL of PAR
• Broad linear dynamic range to 1,000 pg/mL
• Reduced inter-assay variability
• Validated assay that measures drug action on PARP in both in vivo and in vitro settings
• 96 test size

What is the HT PARP In Vivo Pharmacodynamic ELISA Kit II.

Immobilized PAR monoclonal antibody in the wells of a 96-well plate captures cellular PAR and PAR attached to proteins. Incubation with a polyclonal PAR detecting antibody, followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of cellular PAR.

Product Specifications

  • Quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts.
  • Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
  • Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
  • Facilitating development of PARP and PARG targeted therapeutics.

Kit Contents

• PAR Standard, 25 pg/µl
• WIL2-NS Cell Lysate Control Standard, Low
• WIL2-NS Cell Lysate Control Standard, Medium
• WIL2-NS Cell Lysate Control Standard, High
• PAR Polyclonal Detecting Antibody
• Goat anti-Rabbit IgG-HRP
• DNase I, 2 Units/µl
• PARP Sample Buffer
• PARP Cell Lysis Reagent
• 100X Magnesium Cation
• PARP Antibody Diluent
• 20% SDS
• Pre-coated white 96-stripwell plate and 5 sealers
• PARP PeroxyGlow A
• PARP PeroxyGlow B

Kit Contents

  • PAR Standard, 25 pg/µl
  • WIL2-NS Cell Lysate Control Standard, Low
  • WIL2-NS Cell Lysate Control Standard, Medium
  • WIL2-NS Cell Lysate Control Standard, High
  • PAR Polyclonal Detecting Antibody
  • Goat anti-Rabbit IgG-HRP
  • DNase I, 2 Units/µl
  • PARP Sample Buffer
  • PARP Cell Lysis Reagent
  • 100X Magnesium Cation
  • PARP Antibody Diluent
  • 20% SDS
  • Pre-coated white 96-stripwell plate and 5 sealers
  • PARP PeroxyGlow A
  • PARP PeroxyGlow B

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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