HT PARP in vivo Pharmacodynamic ELISA Kit II
HT PARP in vivo Pharmacodynamic ELISA Kit II Summary
• Pre-coated 96 well capture antibody plates
• High signal to noise ratio
• Detection sensitivity of 2 pg/mL of PAR
• Broad linear dynamic range to 1,000 pg/mL
• Reduced inter-assay variability
• Validated assay that measures drug action on PARP in both in vivo and in vitro settings
• 96 test size
What is the HT PARP In Vivo Pharmacodynamic ELISA Kit II.
Immobilized PAR monoclonal antibody in the wells of a 96-well plate captures cellular PAR and PAR attached to proteins. Incubation with a polyclonal PAR detecting antibody, followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of cellular PAR.
- Quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts.
- Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
- Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
- Facilitating development of PARP and PARG targeted therapeutics.
• PAR Standard, 25 pg/µl
• WIL2-NS Cell Lysate Control Standard, Low
• WIL2-NS Cell Lysate Control Standard, Medium
• WIL2-NS Cell Lysate Control Standard, High
• PAR Polyclonal Detecting Antibody
• Goat anti-Rabbit IgG-HRP
• DNase I, 2 Units/µl
• PARP Sample Buffer
• PARP Cell Lysis Reagent
• 100X Magnesium Cation
• PARP Antibody Diluent
• 20% SDS
• Pre-coated white 96-stripwell plate and 5 sealers
• PARP PeroxyGlow A
• PARP PeroxyGlow B
For research use only. Not for diagnositic use.
Measurement of PAR Levels in Jurkat Cells. The HT PARP In Vivo Pharmacodynamic Assay II (Catalog # 4520-096-K) was used to measure poly (ADP-ribose) (PAR) levels in cellular extracts from untreated Jurkat human acute T cell leukemia cells, Jurkat cells treated with PJ34, a potent PARP inhibitor, for 1.5 hours, and in Jurkat cell extracts incubated for 30 minutes with PAR glycohydrolase (PARG), an enzyme that digests PAR.
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