HTF9C Antibody (2U3W8)
Novus Biologicals | Catalog # NBP3-15244
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2U3W8 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HTF9C (Q8IZ69). MSENLDNEGPKPMESCGQESSSALSCPTVSVPPAAPAALEEVEKEGAGAATGPGPQPGLYSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HTF9C Antibody (2U3W8)
Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244]
Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244] - Western blot analysis of extracts of various cell lines, using HTF9C antibody (NBP3-15244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244] -
Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244] - Western blot analysis of K-562, using HTF9C Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244] -
Western Blot: HTF9C Antibody (2U3W8) [NBP3-15244] - Western blot analysis of MCF7, using HTF9C Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for HTF9C Antibody (2U3W8)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HTF9C
Alternate Names
HTF9CHpaII tiny fragments locus 9c protein, MGC102728, TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae), tRNA (uracil-5-)-methyltransferase homolog A, tRNA methyltransferase 2 homolog A
Gene Symbol
TRMT2A
Additional HTF9C Products
Product Documents for HTF9C Antibody (2U3W8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HTF9C Antibody (2U3W8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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