Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2157A
View all 5-HT6 Primary Antibodies »

Product Specifications

Immunogen

Human 5-HT6 peptide
Accession # P50406

Specificity

Detects human 5-HT6 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human 5-HT6 Antibody

5-HT6 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

5-HT6 in Rat Brain.

5-HT6 was detected in perfusion fixed frozen sections of rat brain (medulla) using Rabbit Anti-Human 5-HT6 Monoclonal Antibody (Catalog # MAB9496) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of neuronal cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human 5-HT6 Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (medulla)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-HT6

5-HT6 is a seven transmembrane G-protein coupled 1 receptor for serotonin, with 93% homology between human and rat, and with highest expression in neurons of the striatum, cortex and hippocampus. Expression in cultured primary astrocytes from rats and glia has also been demonstrated for most brain regions. Expression in lymph node, spleen and testis has also been observed. High affinity for psychotropic drugs including tricyclic antidepressants and antipsychotics and high expression in the limbic regions suggest a role for 5-HT6 in emotional behaviors and mental illness. Lower expression of 5-HT6 in cortical layers I-V have been observe in Alzheimer's Disease brains.

Long Name

5-Hydroxytryptamine Receptor 6

Alternate Names

HTR6

Entrez Gene IDs

3362 (Human); 15565 (Mouse); 64354 (Rat)

Gene Symbol

HTR6

UniProt

P50406 (Human)

Additional 5-HT6 Products

Product Documents for Human 5-HT6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 5-HT6 Antibody

For research use only

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Protocols

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