Donkey anti-Rabbit IgG Secondary Antibody [NL557]

R&D Systems | Catalog # NL004

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
R&D Systems

Key Product Details

Species Reactivity

Rabbit

Applications

Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Rabbit IgG (H+L)

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry.

Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

Application
Recommended Usage

Immunocytochemistry

Use at a 1:200 dilution

Reviewed Applications

Read 1 review rated 4 using NL004 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

For research use only

Citations for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

Customer Reviews for Donkey anti-Rabbit IgG Secondary Antibody [NL557] (1)

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  • Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: RT4-D6P2T rat schwannoma cell line
    Species: Human
    Verified Customer | Posted 01/14/2019
    RT4 cell line with Primary snail-rabbit
    Donkey anti-Rabbit IgG Secondary Antibody [NL557] NL004

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FAQs for Donkey anti-Rabbit IgG Secondary Antibody [NL557]

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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