Rabbit IgG NorthernLights™ NL557-conjugated Antibody

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL004

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry.
1 Image
Product Details
Citations (23)
FAQs
Supplemental Products
Reviews (1)

Rabbit IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry. Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

You must select a language.

x

Citations for Rabbit IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification
    Authors: GAE Pickering, F Felix-Ilem, MJ Clark, K Kocsy, J Simpson, I Bellantuon, A Gartland, JM Wilkinson, K Hatzikotou, E Kiss-Toth
    International Journal of Molecular Sciences, 2022-08-16;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation of iPSC line (GLNNFi001-A) from peripheral blood mononuclear cells of a patient with macular corneal dystrophy
    Authors: K Chakrabart, KN Prashanthi, R Shetty, S Argulwar, N Jeyabalan, A Ghosh
    Stem Cell Research, 2022-04-22;62(0):102789.
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: ICC
  3. Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in mice
    Authors: J Li, MD Chordia, Y Zhang, H Zong, D Pan, Z Zuo
    Theranostics, 2022-03-21;12(7):3024-3044.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Metabolic reprogramming mediates hippocampal microglial M1 polarization in response to surgical trauma causing perioperative neurocognitive disorders
    Authors: G Luo, X Wang, Y Cui, Y Cao, Z Zhao, J Zhang
    Journal of Neuroinflammation, 2021-11-13;18(1):267.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice
    Authors: D Halperin, A Stavsky, R Kadir, M Drabkin, O Wormser, Y Yogev, V Dolgin, R Proskorovs, Y Perez, H Nudelman, O Stoler, B Rotblat, T Lifschytz, A Lotan, G Meiri, D Gitler, OS Birk
    Nature Communications, 2021-10-26;12(1):6187.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Knockdown of Ptprn-2 delays the onset of puberty in female rats
    Authors: T Kang, J Ye, P Qin, H Li, Z Yao, Y Liu, Y Ling, Y Zhang, T Yu, H Cao, Y Li, J Wang, F Fang
    Theriogenology, 2021-09-28;176(0):137-148.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Viability of diffuse large B-cell lymphoma cells is regulated by kynurenine 3-monooxygenase activity
    Authors: N Morita, M Hoshi, T Hara, S Ninomiya, T Enoki, M Yoneda, H Tsurumi, K Saito
    Oncology Letters, 2021-09-17;22(5):790.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Short lifespan of syngeneic transplanted MSC is a consequence of in vivo apoptosis and immune cell recruitment in mice
    Authors: MB Preda, CA Neculachi, IM Fenyo, AM Vacaru, MA Publik, M Simionescu, A Burlacu
    Cell Death & Disease, 2021-06-02;12(6):566.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Identification, presence, and possible multifunctional regulatory role of invertebrate gonadotropin-releasing hormone/corazonin molecule in the great pond snail (Lymnaea stagnalis)
    Authors: I Fodor, Z Zrinyi, R Horváth, P Urbán, R Herczeg, G Büki, JM Koene, PS Tsai, Z Pirger
    Gen Comp Endocrinol, 2020-09-20;299(0):113621.
    Species: Snail
    Sample Types: Whole Tissue
    Applications: IHC
  10. Radiation Response of Murine Embryonic Stem Cells
    Authors: CE Hellweg, V Shinde, SP Srinivasan, M Henry, T Rotshteyn, C Baumstark-, C Schmitz, S Feles, LF Spitta, R Hemmersbac, J Hescheler, A Sachinidis
    Cells, 2020-07-09;9(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice
    Authors: C Tashita, M Hoshi, A Hirata, K Nakamoto, T Ando, T Hattori, Y Yamamoto, H Tezuka, H Tomita, A Hara, K Saito
    World J. Gastroenterol., 2020-03-07;26(9):918-932.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence
    Authors: T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan
    J. Biol. Chem., 2019-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Subcellular Peptide Localization in Single Identified Neurons by Capillary Microsampling Mass Spectrometry
    Authors: L Zhang, N Khattar, I Kemenes, G Kemenes, Z Zrinyi, Z Pirger, A Vertes
    Sci Rep, 2018-08-15;8(1):12227.
    Species: Snail
    Sample Types: Whole Tissue
    Applications: IHC
  14. Chronic PARP-1 inhibition reduces carotid vessel remodeling and oxidative damage of the dorsal hippocampus in spontaneously hypertensive rats
    Authors: K Eros, K Magyar, L Deres, A Skazel, A Riba, Z Vamos, T Kalai, F Gallyas, B Sumegi, K Toth, R Halmosi
    PLoS ONE, 2017-03-24;12(3):e0174401.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons.
    Authors: Shulman Y, Stavsky A, Fedorova T, Mikulincer D, Atias M, Radinsky I, Kahn J, Slutsky I, Gitler D
    J Neurosci, 2015-01-21;35(3):985-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  16. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
    Authors: Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
    Development, 2014-09-01;141(17):3319-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Alteration in the Wnt microenvironment directly regulates molecular events leading to pulmonary senescence.
    Authors: Kovacs T, Csongei V, Feller D, Ernszt D, Smuk G, Sarosi V, Jakab L, Kvell K, Bartis D, Pongracz J
    Aging Cell, 2014-07-01;13(5):838-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Perioperative aspirin improves neurological outcome after focal brain ischemia possibly via inhibition of Notch 1 in rat.
    Authors: Wang Z, Huang W, Zuo Z
    J Neuroinflammation, 2014-03-25;11(0):56.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Intercellular transport of Oct4 in mammalian cells: a basic principle to expand a stem cell niche?
    Authors: Rolf HJ, Niebert S, Niebert M, Gaus L, Schliephake H, Wiese KG
    PLoS ONE, 2012-02-16;7(2):e32287.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Inhibition of exocytosis or endocytosis blocks activity-dependent redistribution of synapsin.
    Authors: Orenbuch A, Ayelet O, Shulman Y, Yoav S, Lipstein N, Noa L, Bechar A, Amit B, Lavy Y, Yotam L, Brumer E, Eliaz B, Vasileva M, Mariya V, Kahn J, Joy K, Barki-Harrington L, Liza BH, Kuner T, Thomas K, Gitler D, Daniel G
    J. Neurochem., 2011-12-02;120(2):248-58.
    Applications: ICC
  22. A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
    Authors: Gopalakrishna-Pillai S, Iverson LE
    PLoS ONE, 2011-06-15;6(6):e20663.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Tumor necrosis factor receptor expression and signaling in renal cell carcinoma.
    Authors: Al-Lamki RS, Sadler TJ, Wang J
    Am. J. Pathol., 2010-06-21;177(2):943-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
View all Antibody FAQs
Loading...

Reviews for Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Rabbit IgG NorthernLights™ NL557-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 01/14/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: RT4-D6P2T rat schwannoma cell line Species: Human

RT4 cell line with Primary snail-rabbit