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Human 5-HT7 APC-conjugated Antibody

Catalog #: FAB10325A Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB10325A-100

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Detection of 5-HT7 in 5-HT7 HEK293/eGFP transfecants vs Irrelevant HEK293/eGFP transfecants by Flow Cytometry
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Human 5-HT7 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human 5-HTR7 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1015322
Purification
Protein A or G purified from cell culture supernatant
Immunogen
 Synthetic peptide containing human 5-HTR7
Accession # P34969
Formulation
Supplied in a saline solution containing Tris, NaCl and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Immunohistochemistry
CyTOF-ready

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry 5-HT7 Antibody (1015322) [Allophycocyanin] View Larger

Detection of 5-HT7 in 5-HT7 HEK293/eGFP transfecants vs Irrelevant HEK293/eGFP transfecants by Flow Cytometry 5-HT7 HEK293/eGFP transfecants (A) vs Irrelevant HEK293/eGFP transfecants (B) were stained with Mouse Anti-Human 5-HT7 APC‑conjugated Monoclonal Antibody (Catalog # FAB10325A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5-HT7

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels.  It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions.  5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Long Name
5-Hydroxytryptamine Receptor 7
Entrez Gene IDs
3363 (Human); 15566 (Mouse); 65032 (Rat)
Alternate Names
5HT7; 5-HT7; 5-HT-7; 5-HT75-hydroxytryptamine receptor 7; 5-HT-X; 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled); HTR7; serotonin 5-HT-7 receptor; Serotonin receptor 7

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