Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 402104
View all A33 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Specificity

Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human A33 APC‑conjugated Antibody

Detection of A33 antibody in HT-29 Human Cell Line antibody by Flow Cytometry.

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 APC-conjugated Monoclonal Antibody (Catalog # FAB3080A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of A33 in Colo205 cells by Flow Cytometry.

Colo205 cells were stained with Rat Anti-Human A33 APC‑conjugated Monoclonal Antibody (Catalog # FAB3080A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human A33 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol

GPA33

UniProt

Q99795

Additional A33 Products

Product Documents for Human A33 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human A33 APC‑conjugated Antibody

For research use only

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