Human ACLP Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6425V-100UG
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Human ACLP Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ACLP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ACLP is observed.
Source
Monoclonal Mouse IgG1 Clone # 590831
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACLP
Glu345-Phe1158
Accession # Q8IUX7
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HASMC human human aortic smooth muscle cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ACLP

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1), is a 175 kDa molecule that contains a discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942). It is expressed in proliferating adipocytes and represses the transcription of FABP4. ACLP promotes macrophage cholesterol retention, the formation of foam cells, and inflammatory responses. It is also an extracellular matrix-associated protein found in fibrotic tissues including vascular smooth muscle. An alternately spliced isoform lacks aa 1-457 and has an altered sequence at its N-terminus. Over aa 345-1158, human ACLP shares 88% aa sequence identity with mouse and rat ACLP.

Long Name
Aortic Carboxypeptidase-like Protein
Entrez Gene IDs
165 (Human)
Alternate Names
ACLP; ACLPadipocyte enhancer binding protein 1; AE binding protein 1; AE-binding protein 1adipocyte enhancer-binding protein 1; AEBP1; Aortic carboxypeptidase-like protein; FLJ33612

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