Human ADAM12 Quantikine ELISA Kit

  (1 citations)     
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Product Details
Assay Procedure
Citations (1)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), Heparin Plasma (25 uL), Urine (50 uL)
  • Sensitivity
    0.076 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human ADAM12. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC34 , Quantikine Immunoassay Control Set 709 for Human ADAM12 - Please Inquire
Product Summary
The Quantikine Human ADAM12 immunoassay is a 4.5 hour solid phase ELISA designed to measure human ADAM12 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human ADAM12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ADAM12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ADAM12.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.39 3.57 6.84 1.43 3.62 6.74
Standard Deviation 0.057 0.131 0.235 0.079 0.145 0.299
CV% 4.1 3.7 3.4 5.5 4 4.4

Recovery

The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 98 88-105
Heparin Plasma (n=4) 97 92-106
Serum (n=4) 98 89-106
Urine (n=4) 99 91-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAM12 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 ADAM12 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ADAM12
Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.
  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 12
  • Entrez Gene IDs:
    8038 (Human)
  • Alternate Names:
    a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Evaluation of ADAM-12 as a Diagnostic Biomarker of Ectopic Pregnancy in Women with a Pregnancy of Unknown Location.
    Authors: Horne AW, Brown JK, Tong S, Kaitu'u-Lino T
    PLoS ONE, 2012;7(8):e41442.
    Species: Human
    Sample Type: Serum

FAQs

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ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 709 for Human ADAM12

Ctrl QC34
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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