Human Aiolos/IKZF3 Antibody

Catalog #: MAB8625 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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MAB8625
MAB8625-SP

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Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aiolos/IKZF3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aiolos/IKZF3 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 914821

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human Aiolos/IKZF3
Met1-Gly54
Accession # Q9UKT9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Complete Your Research

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Complete Your Experiment

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Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aiolos/IKZF3

IKAROS family zinc finger 3 (IKZF3), also known as Aiolos, is a hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation.

Long Name

DNA-binding Protein Aiolos

Entrez Gene IDs

22806 (Human); 22780 (Mouse); 303511 (Rat)

Alternate Names

AIO; Aiolos; IKAROS family zinc finger 3 (Aiolos); Ikaros family zinc finger protein 3; IKZF3; zinc finger protein Aiolos; ZNFN1A3; ZNFN1A3subfamily 1A, 3 (Aiolos)