Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 914821
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Product Specifications

Immunogen

E. coli-derived recombinant human Aiolos/IKZF3
Met1-Gly54
Accession # Q9UKT9

Specificity

Detects human Aiolos/IKZF3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Aiolos/IKZF3 Antibody

Detection of Aiolos/IKZF3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Aiolos/IKZF3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Applications for Human Aiolos/IKZF3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aiolos/IKZF3

IKAROS family zinc finger 3 (IKZF3), also known as Aiolos, is a hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation.

Long Name

DNA-binding Protein Aiolos

Alternate Names

AIO, IKZF3, ZNFN1A3

Entrez Gene IDs

22806 (Human); 22780 (Mouse); 303511 (Rat)

Gene Symbol

IKZF3

UniProt

Additional Aiolos/IKZF3 Products

Product Documents for Human Aiolos/IKZF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Aiolos/IKZF3 Antibody

For research use only

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