Human Allergin-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7638G-100UG
Detection  of Allergin‑1 in Human Blood-Derived Monocytes by Flow Cytometry.
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Human Allergin-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Allergin-1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 767727
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Allergin-1
Met1-Lys227
Accession # Q7Z6M3
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Allergin‑1 in Human Blood-Derived Monocytes by Flow Cytometry. View Larger

Detection of Allergin‑1 in Human Blood-Derived Monocytes by Flow Cytometry. Human blood-derived monocytes were stained with Mouse Anti-Human Allergin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7638G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Allergin-1

Allergin-1, also known as MCA32 and c17orf60 is a 70-72 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils, dendritic cells, B cells and monocytes, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilon RI. Upon IgE mediated Fc epsilon RI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilon RI tetrameric complex, dampening IgE receptor downstream signaling and abrogating mast cell degranulation. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40-42 kDa splice variants exist that show deletions of one of the two Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 123-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. It would appear that mouse Allergin-1 exists in two forms; a soluble form lacking the TM segment, and a TM form that is analogous to the human 1S1 isoform (no two Ig-like domain-containing isoform exists). Within this context (aa 20-122 plus aa 218-227 in human), human and mouse share 48% aa sequence identity.

Long Name
Allergy Inhibitory Receptor 1
Entrez Gene IDs
284021 (Human); 380732 (Mouse)
Alternate Names
Allergin1; Allergin-1; Allergy Inhibitory Receptor 1; C17orf60; Chromosome 17 Open Reading Frame 60; Mast Cell Antigen 32; Mast Cell Immunoglobulin-Like Receptor 1; MCA32; MCA-32; MCA32-1S2; MILR1; Probable Mast Cell Antigen 32 Homolog

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