Detection of Allergin‑1 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # MAB7638) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Allergin‑1 in U‑87 MG Human Cell Line.
Allergin‑1 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # MAB7638) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MCA32 (Mast Cell Antigen; also Allergin‑1 and c17orf60) is a 70 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils and macrophages, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilon RI. Upon IgE mediated Fc epsilon RI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilon RI tetrameric complex, dampening IgE receptor downstream signaling. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40 kDa splice variants exist that show deletions of the Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 128-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. This second isoform represents the antigen used for immunization in this product (GenBank BAJ08253). Over aa 29-131, the Allergin‑1S2 isoform shares 100% sequence identity with human Allergin‑1L full length isoform and only 24% aa sequence identity with mouse Allergin‑1.
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