Human Aminopeptidase N/CD13 Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Aminopeptidase N/CD13 in flow cytometry
  • Source
    Monoclonal Mouse IgG1 Clone # WM-15
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human AML cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Aminopeptidase N/CD13 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Aminopeptidase N/CD13 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8284N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Aminopeptidase N/CD13

The human ANPEP gene encodes Aminopeptidase N (APN), which is also known as Microsomal Aminopeptidase, Alanyl Aminopeptidase, Aminopeptidase M, CD13, or Membrane Protein p161 (1-3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

  • References:
    1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
    2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
    3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.
  • Entrez Gene IDs:
    290 (Human); 16790 (Mouse)
  • Alternate Names:
    alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN
Related Research Areas

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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