Human Amyloid beta (aa1-40) Quantikine ELISA Kit

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  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Tissue Lysates (25 uL), Cerebrospinal Fluid (25 uL)
  • Sensitivity
    8.17 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
  • Specificity
    Natural human Amyloid beta (aa1-40).
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC216 , Quantikine Immunoassay Control Set 883 Human Amyloid beta - Please Inquire
Product Summary
The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 58.9 264 556 56.3 247 518
Standard Deviation 4.23 5.76 11.3 4.31 22.4 30.5
CV% 7.2 2.2 2 7.7 9.1 5.9

Recovery

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 95-105
Cell Lysis Buffer (n=2) 99 91-110
Cerebrospinal Fluid (n=4) 104 96-113
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
 beta Amyloid [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
  • Entrez Gene IDs:
    351 (Human); 11820 (Mouse)
  • Alternate Names:
    Abeta; Amyloid beta; beta-Amyloid Peptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Sample Type
  1. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
    Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  2. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
    Sci Rep, 2016;6(0):37486.
    Species: Human
    Sample Type: Serum

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ELISA Controls
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Quantikine Immunoassay Control Set 883 Human Amyloid beta

Ctrl QC216  
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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