Human Amyloid beta (aa1-40) Quantikine ELISA Kit

(2 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Cerebrospinal Fluid (25 uL)
  • Sensitivity
    0.147 ng/mL
  • Assay Range
    0.8 - 50 ng/mL (Cell Culture Supernates, Cerebrospinal Fluid)
  • Specificity
    Natural human Amyloid beta (aa1-40).
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC216, Quantikine Immunoassay Control Set 883 Human Amyloid beta - Please Inquire
Product Summary
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Amyloid beta (aa1-40) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Amyloid beta (aa1-40) present is bound by the immobilized antibody. After washing away any unbound substances, an
enzyme-linked monoclonal antibody specific for human Amyloid beta (aa1-40) is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Amyloid beta (aa1-40) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cerebrospinal Fluid
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean5.1415.931.84.9914.929.6
Standard Deviation0.1770.9172.160.2981.212.72
CV%3.45.86.868.19.2

Recovery

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 94 80-108
Cerebrospinal Fluid (n=4) 91 83-99
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Human Amyloid beta (aa1-40) Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
    • Entrez Gene IDs
      351 (Human); 11820 (Mouse);
    • Alternate Names
      Abeta; Amyloid beta; beta-Amyloid Peptide;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    2 Citations: Showing 1 - 2
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    Species
    Sample Type
    1. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
      Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
      Aging Cell, 2017;0(0):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
      Sci Rep, 2016;6(0):37486.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 883 Human Amyloid beta

    Ctrl QC216
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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