Best Seller

Human Amyloid beta (aa1-40) Quantikine ELISA Kit

R&D Systems | Catalog # DAB140B

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 µL), Tissue Lysates (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

8.17 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
View all Amyloid beta ELISA Kits »

Product Summary for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural human Amyloid beta (aa1-40).

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cerebrospinal Fluid, Tissue Lysates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 58.9 264 556 56.3 247 518
Standard Deviation 4.23 5.76 11.3 4.31 22.4 30.5
CV% 7.2 2.2 2.0 7.7 9.1 5.9

Recovery for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 95-105
Cell Lysis Buffer (n=2) 99 91-110
Cerebrospinal Fluid (n=4) 104 96-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.

Human Amyloid beta (aa 1-40) ELISA Linearity
Human Amyloid beta (aa 1-40) ELISA Linearity

Scientific Data Images for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

Human Amyloid beta (aa 1-40) ELISA Standard Curve

Human Amyloid beta (aa 1-40) ELISA Standard Curve

Human Amyloid beta (aa 1-40) ELISA Standard Curve

Human Amyloid beta (aa 1-40) ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

Additional Amyloid beta Products

Product Documents for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

Customer Reviews for Human Amyloid beta (aa1-40) Quantikine ELISA Kit (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Amyloid beta (aa1-40) Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Works good for human plasma
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Species: Human
    Verified Customer | Posted 04/08/2026
    Standard curve.
    Created STD concentration from 0-1000 pg/ml, works good to test A beta 1-40 in human plasma.
    Human Amyloid beta (aa1-40) Quantikine ELISA Kit DAB140B

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits