Human Angiopoietin-1 Quantikine ELISA Kit

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Human Angiopoietin-1 ELISA Calibrator Diluent RD5P (1X) Standard Curve
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Human Angiopoietin-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sensitivity
10.3 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human Angiopoietin-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Angiopoietin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 611 1217 2502 619 1223 2496
Standard Deviation 20.8 24.2 67 42.4 71.5 113
CV% 3.4 2 2.7 6.8 5.8 4.5

Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 587 1179 2339 620 1230 2533
Standard Deviation 14 28.5 76.1 39.5 67.1 141
CV% 2.4 2.4 3.3 6.4 5.5 5.6

Recovery

The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 97-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Angiopoietin-1 ELISA Linearity

Data Examples

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P (1X) Standard Curve

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P Concentrate Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: B7-H3

B7-H3 is an immunoglobulin superfamily protein that is expressed by osteoblasts, fibroblasts, ciliated respiratory epithelial cells, anterior pituitary progenitor cells, first trimester extravillous trophoblasts, and multiple tumor cells. It is induced on activated dendritic cells (DC), skeletal muscle myocytes, and monocytes. B7-H3 can both stimulate and inhibit immune cell activation. As a co-stimulator, B7-H3 augments macrophage activation and promotes proliferation and cytokine secretion by CD4+ and CD8+ T cells. As a co-inhibitor, tumor cell B7-H3 blocks the cytolytic activity of NK cells, limits cytokine production by activated T cells, and imparts an immunosuppressive phenotype to dendritic cells. B7-H3 can interact with TREML2/TLT-2 to enhance T cell activation including the CD8+ T cell-mediated lysis of tumor cells.

Long Name:
B7 Homolog 3
Entrez Gene IDs:
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names:
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Angiopoietin-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
    Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
    Leukemia, 2021;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Defined MSC exosome with high yield and purity to improve regenerative activity
    Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
    Journal of tissue engineering, 2021;12(0):2041731421100.
    Species: Human
    Sample Types: Cell Lysates
  3. Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway
    Authors: S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto
    Journal of dermatological science, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
    Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
    The Journal of nutritional biochemistry, 2021;0(0):108626.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
    Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
    Aging (Albany NY), 2019;11(24):12147-12164.
    Species: Human
    Sample Types: Serum
  6. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk,
    Kidney Int Rep, 2019;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  7. MicroRNA-411 and Its 5'-IsomiR Have Distinct Targets and Functions and Are Differentially Regulated in the Vasculature under Ischemia
    Authors: RVCT van der Kw, T Woudenberg, PHA Quax, AY Nossent
    Mol. Ther., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
    Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
    Crit Care, 2019;23(1):117.
    Species: Human
    Sample Types: Plasma
  9. Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
    Authors: M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes
    Stem Cells Int, 2019;2019(0):1916542.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
    Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
    BMC Nephrol, 2018;19(1):119.
    Species: Human
    Sample Types: Serum
  11. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis
    Authors: L Desbourdes, J Javary, T Charbonnie, N Ishac, J Bourgeais, A Iltis, JC Chomel, A Turhan, F Guilloton, K Tarte, MV Demattei, E Ducrocq, F Rouleux-Bo, E Gyan, O Hérault, J Domenech
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Tie2 as a novel key factor of microangiopathy in systemic sclerosis
    Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
    Arthritis Res. Ther., 2017;19(1):105.
    Species: Human
    Sample Types: Serum
  14. Circulating Angiopoietin-1 Is Not a Biomarker of Disease Severity or Prognosis in Pulmonary Hypertension
    PLoS ONE, 2016;11(11):e0165982.
    Species: Human
    Sample Types: Plasma
  15. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016;2(4):e1501290.
    Species: Human
    Sample Types: Cell Lysates
  16. Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signalling
    Authors: David O Bates
    J. Physiol. (Lond.), 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
    Sci Rep, 2016;6(0):39400.
    Species: Human
    Sample Types: Serum
  19. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
    Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
    PLoS ONE, 2015;10(8):e0136205.
    Species: Human
    Sample Types: Plasma
  20. EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
    Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
    Stem Cells, 2015;33(9):2838-49.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
    Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
    BMC Cancer, 2014;14(0):696.
    Species: Human
    Sample Types: Serum
  22. Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
    Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
    PLoS ONE, 2014;9(10):e109246.
    Species: Human
    Sample Types: Serum
  23. Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
    Proc Natl Acad Sci U S A, 2012;109(25):10024-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  24. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
    Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
    BMC Cancer, 2011;11(0):286.
    Species: Human
    Sample Types: Serum
  25. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
    Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
    Diabetologia, 2011;54(9):2358-67.
    Species: Human
    Sample Types: Serum
  26. CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
    Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
    J. Neurosci. Res., 2011;89(7):979-85.
    Species: Human
    Sample Types: Serum
  27. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
    Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(6):L849-56.
    Species: Human
    Sample Types: Tissue Homogenates
  28. Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
    Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
    Fertil. Steril., 2010;93(5):1493-9.
    Species: Human
    Sample Types: Serum
  29. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
    Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
    Ann. Hematol., 2009;88(7):667-71.
    Species: Human
    Sample Types: Plasma
  30. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
    Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
    Exp. Hematol., 2009;37(6):673-8.
    Species: Human
    Sample Types: Plasma
  31. Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
    Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
    Ann. Surg., 2009;249(6):973-7.
    Species: Human
    Sample Types: Plasma
  32. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
    Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
    Ann. Surg., 2008;247(2):320-6.
    Species: Human
    Sample Types: Plasma
  33. Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
    Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
    J. Mol. Cell. Cardiol., 2008;45(1):70-80.
    Species: Porcine
    Sample Types: Tissue Homogenates
  34. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
    Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
    Br. J. Cancer, 2007;97(7):877-82.
    Species: Human
    Sample Types: Plasma
  36. Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
    Authors: Choudhury A, Freestone B, Patel J, Lip GY
    Chest, 2007;132(6):1913-9.
    Species: Human
    Sample Types: Plasma
  37. Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
    Authors: Kanazawa H, Nomura S, Asai K
    Chest, 2007;131(4):1035-41.
    Species: Human
    Sample Types: Sputum
  38. Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
    Authors: Kanazawa H, Tochino Y, Asai K
    J. Allergy Clin. Immunol., 2007;121(2):390-5.
    Species: Human
    Sample Types: Sputum
  39. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
    Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
    Br. J. Haematol., 2007;137(6):560-8.
    Species: Human
    Sample Types: Serum
  40. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007;25(8):1862-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
    Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
    Cancer Lett., 2007;248(1):131-6.
    Species: Human
    Sample Types: Plasma
  42. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
    Authors: Caine GJ, Ryan P, Lip GY, Blann AD
    Cancer Lett., 2007;251(2):296-301.
    Species: Human
    Sample Types: Plasma
  43. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
    Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
    Cytokine, 2006;36(5):291-5.
    Species: Human
    Sample Types: Serum
  44. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
    Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
    Crit. Care Med., 0;0(0):.
    Species: Human
    Sample Types: Plasma

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Human Angiopoietin-1 Quantikine ELISA Kit
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Human Angiopoietin-1 Quantikine ELISA Kit
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Application: Sample Tested: Cell Culture Supernates