Human Angiopoietin-1 Quantikine ELISA Kit

R&D Systems | Catalog # DANG10

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Platelet-poor Citrate Plasma (10 µL), Saliva (25 µL), Human Milk (25 µL)

Sensitivity

10.3 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Saliva, Human Milk)
View all Angiopoietin-1 ELISA Kits »

Product Summary for Human Angiopoietin-1 Quantikine ELISA Kit

The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Angiopoietin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Angiopoietin-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Human Milk, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 611 1217 2502 619 1223 2496
Standard Deviation 20.8 24.2 67.0 42.4 71.5 113
CV% 3.4 2.0 2.7 6.8 5.8 4.5

EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 587 1179 2339 620 1230 2533
Standard Deviation 14.0 28.5 76.1 39.5 67.1 141
CV% 2.4 2.4 3.3 6.4 5.5 5.6

Recovery for Human Angiopoietin-1 Quantikine ELISA Kit

The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 97-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Angiopoietin-1 ELISA Linearity

Scientific Data Images for Human Angiopoietin-1 Quantikine ELISA Kit

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P (1X) Standard Curve

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P (1X) Standard Curve

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P Concentrate Standard Curve

Human Angiopoietin-1 ELISA Calibrator Diluent RD5P Concentrate Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It signals through the receptor tyrosine kinase Tie-2 and binds a variety of integrins and the extracellular matrix. Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. It can suppress several VEGF-induced effects on the vasculature. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis.

Alternate Names

ANGPT1

Entrez Gene IDs

284 (Human); 11600 (Mouse)

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Human Angiopoietin-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Angiopoietin-1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Angiopoietin-1 Quantikine ELISA Kit

Customer Reviews for Human Angiopoietin-1 Quantikine ELISA Kit (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Angiopoietin-1 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Angiopoietin-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: pericytes
    Verified Customer | Posted 04/30/2019
  • Human Angiopoietin-1 Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 10/14/2016
    Human Angiopoietin-1 Quantikine ELISA Kit DANG10

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

View specific protocols for Human Angiopoietin-1 Quantikine ELISA Kit (DANG10):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits