Human Angiopoietin-1 Quantikine ELISA Kit

(33 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Platelet-poor Citrate Plasma (10 uL), Saliva (25 uL), Human Milk (25 uL)
  • Sensitivity
    10.3 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Saliva, Human Milk)
  • Specificity
    Natural and recombinant human Angiopoietin-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC42, Quantikine Immunoassay Control Set 841 Human Angiopoietin-1 - Please Inquire
Product Summary
The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Angiopoietin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean6111217250261912232496
Standard Deviation20.824.26742.471.5113
CV%3.422.76.85.84.5

Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean5871179233962012302533
Standard Deviation1428.576.139.567.1141
CV%2.42.43.36.45.55.6

Recovery

The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 97-110
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-1 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Angiopoietin-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-1
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
    • Entrez Gene IDs
      284 (Human); 11600 (Mouse);
    • Alternate Names
      AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    33 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Tie2 as a novel key factor of microangiopathy in systemic sclerosis
      Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
      Arthritis Res. Ther., 2017;19(1):105.
      Species: Human
      Sample Type: Serum
    2. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
      Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
      Lab. Invest., 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
      Sci Rep, 2016;6(0):39400.
      Species: Human
      Sample Type: Serum
    4. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
      Sci Adv, 2016;2(4):e1501290.
      Species: Human
      Sample Type: Cell Lysates
    5. Circulating Angiopoietin-1 Is Not a Biomarker of Disease Severity or Prognosis in Pulmonary Hypertension
      PLoS ONE, 2016;11(11):e0165982.
      Species: Human
      Sample Type: Plasma
    6. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
      Sci Rep, 2016;6(0):29029.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
      Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
      PLoS ONE, 2015;10(8):e0136205.
      Species: Human
      Sample Type: Plasma
    8. EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
      Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
      Stem Cells, 2015;33(9):2838-49.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
      Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
      BMC Cancer, 2014;14(0):696.
      Species: Human
      Sample Type: Serum
    10. Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
      Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
      PLoS ONE, 2014;9(10):e109246.
      Species: Human
      Sample Type: Serum
    11. Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
      Proc Natl Acad Sci U S A, 2012;109(25):10024-9.
      Species: Primate - Papio cynocephalus (Yellow Baboon)
      Sample Type: Serum
    12. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
      Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
      BMC Cancer, 2011;11(0):286.
      Species: Human
      Sample Type: Serum
    13. CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
      Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
      J. Neurosci. Res., 2011;89(7):979-85.
      Species: Human
      Sample Type: Serum
    14. Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
      Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
      Diabetologia, 2011;54(9):2358-67.
      Species: Human
      Sample Type: Serum
    15. Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
      Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
      Fertil. Steril., 2010;93(5):1493-9.
      Species: Human
      Sample Type: Serum
    16. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
      Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
      Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(6):L849-56.
      Species: Human
      Sample Type: Tissue Homogenates
    17. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
      Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
      Exp. Hematol., 2009;37(6):673-8.
      Species: Human
      Sample Type: Plasma
    18. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
      Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
      Ann. Hematol., 2009;88(7):667-71.
      Species: Human
      Sample Type: Plasma
    19. Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
      Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
      Ann. Surg., 2009;249(6):973-7.
      Species: Human
      Sample Type: Plasma
    20. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
      Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
      Ann. Surg., 2008;247(2):320-6.
      Species: Human
      Sample Type: Plasma
    21. Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
      Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
      J. Mol. Cell. Cardiol., 2008;45(1):70-80.
      Species: Porcine
      Sample Type: Tissue Homogenates
    22. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
      Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
      Br. J. Cancer, 2007;97(7):877-82.
      Species: Human
      Sample Type: Plasma
    24. Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
      Authors: Kanazawa H, Tochino Y, Asai K
      J. Allergy Clin. Immunol., 2007;121(2):390-5.
      Species: Human
      Sample Type: Sputum
    25. Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
      Authors: Choudhury A, Freestone B, Patel J, Lip GY
      Chest, 2007;132(6):1913-9.
      Species: Human
      Sample Type: Plasma
    26. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
      Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
      Br. J. Haematol., 2007;137(6):560-8.
      Species: Human
      Sample Type: Serum
    27. Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
      Authors: Kanazawa H, Nomura S, Asai K
      Chest, 2007;131(4):1035-41.
      Species: Human
      Sample Type: Sputum
    28. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
      Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
      Cancer Lett., 2007;248(1):131-6.
      Species: Human
      Sample Type: Plasma
    29. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
      Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
      Stem Cells, 2007;25(8):1862-71.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
      Authors: Caine GJ, Ryan P, Lip GY, Blann AD
      Cancer Lett., 2007;251(2):296-301.
      Species: Human
      Sample Type: Plasma
    31. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
      Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
      Cytokine, 2006;36(5):291-5.
      Species: Human
      Sample Type: Serum
    32. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
      Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
      Crit. Care Med., 0;0(0):.
      Species: Human
      Sample Type: Plasma
    Expand to show all 33 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 841 Human Angiopoietin-1

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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

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