Human APLP-2 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF4945X Datasheet / COA / SDS

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AF4945X-100UG

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Human APLP-2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) APLP-2 is observed and less than 1% cross-reactivity with recombinant human APLP-1 and rmAPLP-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human APLP-2
Leu37-Ser680
Accession # AAH00373

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APLP-2

APLP-2 is a 100-170 kDa glycoprotein, member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% amino acid sequence identity with mouse and rat APLP-2, respectively.

Long Name

Amyloid Beta [A4] Precursor-like Protein 2

Entrez Gene IDs

334 (Human); 11804 (Mouse); 64312 (Rat)

Alternate Names

amyloid beta (A4) precursor-like protein 2; amyloid precursor protein homolog HSD-2; Amyloid protein homolog; amyloid-like protein 2; APLP2; APLP-2; APPH; APPHCDEI box-binding protein; APPL2; CDEBP; WALPLP2; YWK-II