Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) APLP-2 is observed and less than 1% cross-reactivity with recombinant human APLP-1 and rmAPLP-1 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human APLP-2 Leu37-Ser680 Accession # AAH00373
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human APLP‑2 by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human APLP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for APLP‑2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
APLP-2 is a 100-170 kDa glycoprotein, member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% amino acid sequence identity with mouse and rat APLP-2, respectively.
Amyloid Beta [A4] Precursor-like Protein 2
Entrez Gene IDs:
334 (Human); 11804 (Mouse); 64312 (Rat)
amyloid beta (A4) precursor-like protein 2; amyloid precursor protein homolog HSD-2; Amyloid protein homolog; amyloid-like protein 2; APLP2; APLP-2; APPH; APPHCDEI box-binding protein; APPL2; CDEBP; WALPLP2; YWK-II
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