Human Aquaporin 4/AQP4 Antibody

R&D Systems | Catalog # MAB11748

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Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, COMET

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3366C
View all Aquaporin 4/AQP4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # P55087

Specificity

Detects a synthetic peptide specific for human Aquaporin-4 around amino acid 315 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Aquaporin 4/AQP4 Antibody

Detection of AQP4 in Human Brain Cortex via seqIF™ staining on COMET™

AQP4 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11748) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH)Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder.

Detection of Aquaporin 4/AQP4 in Human Brain Cortex.

Aquaporin 4/AQP4 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Rabbit Anti-Human Aquaporin 4/AQP4 Monoclonal Antibody (Catalog # MAB11748) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Aquaporin 4/AQP4 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex

Multiplex Immunofluorescence

10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Aquaporin 4/AQP4

Aquaporin 4, AQP4, is a 35 kDa water channel protein that plays an important role in brain water homeostasis.  AQP4 is highly expressed in the end feet of astrocytes in the brain and CNS with the largest presence in the cerebellum and spinal cord grey matter.  It can also be located in epithelial cells of many organs throughout the body.  The main function of AQP4 is to provide fast water transportation and homeostatic balance throughout the central nervous system.

References

  1. Jung JS, Bhat RV, Preston GM, Guggino WB, Baraban JM, Agre P. Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):13052-6. doi: 10.1073/pnas.91.26.13052. PMID: 7528931; PMCID: PMC45579. 
  2. Halsey AM, Conner AC, Bill RM, Logan A, Ahmed Z. Aquaporins and Their Regulation after Spinal Cord Injury. Cells. 2018 Oct 18;7(10):174. doi: 10.3390/cells7100174. PMID: 30340399; PMCID: PMC6210264. 
  3. Verkman AS, Phuan PW, Asavapanumas N, Tradtrantip L. Biology of AQP4 and anti-AQP4 antibody: therapeutic implications for NMO. Brain Pathol. 2013 Nov;23(6):684-95. doi: 10.1111/bpa.12085. PMID: 24118484; PMCID: PMC3890327. 
  4. Gleiser C, Wagner A, Fallier-Becker P, Wolburg H, Hirt B, Mack AF. Aquaporin-4 in Astroglial Cells in the CNS and Supporting Cells of Sensory Organs-A Comparative Perspective. Int J Mol Sci. 2016 Aug 26;17(9):1411. doi: 10.3390/ijms17091411. PMID: 27571065; PMCID: PMC5037691.

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Entrez Gene IDs

361 (Human)

Gene Symbol

AQP4

UniProt

P55087 (Human)

Additional Aquaporin 4/AQP4 Products

Product Documents for Human Aquaporin 4/AQP4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Aquaporin 4/AQP4 Antibody

For research use only

Related Research Areas

Ion Channels and Regulators

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Protocols

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