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Human ATG4B Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB5279T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5279T-100UG

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Human ATG4B Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ATG4B/Apg4b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4A is observed.
Source
Monoclonal Mouse IgG2b Clone # 515803
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4B/Apg4b
Asp2-Leu393 (predicted)
Accession # Q9Y4P1
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ATG4B

Autophagy related protein 4 homolog B (ATG4B), also known as autophagin 1 and Apg4b, is a cytosolic cysteine protease that is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. Human ATG4B shares 98% and 95% amino acid sequence identity with chimpanzee and mouse ATG4B, respectively.

Long Name
ATG4 Autophagy Related 4 Homolog B
Entrez Gene IDs
23192 (Human); 66615 (Mouse); 316640 (Rat)
Alternate Names
APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943

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