Human B7‑H4 PE‑conjugated Antibody

R&D Systems | Catalog # FAB6576P

R&D Systems
Discontinued Product
FAB6576P has been discontinued. View all B7-H4 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 935317
View all B7-H4 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human B7-H4
Phe29-Ser258
Accession # Q7Z7D3

Specificity

Stains human B7-H4 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human B7‑H4 PE‑conjugated Antibody

Detection of B7-H4 antibody in HEK293 Human Cell Line Transfected with Human B7-H4 and eGFP antibody by Flow Cytometry.

Detection of B7‑H4 in HEK293 Human Cell Line Transfected with Human B7-H4 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human B7-H4 and eGFP were stained with either (A) Mouse Anti-Human B7-H4 PE-conjugated Monoclonal Antibody (Catalog # FAB6576P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human B7‑H4 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human B7-H4 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H4

B7-H4, also known as VTCN1, B7x and B7S1, is a 50‑80 kDa glycosylated member of the BTN/MOG family of immunomodulatory protein (1, 2). Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail (3-5). Within the ECD, human B7-H4 shares 90% aa sequence identity with mouse and rat B7-H4. It shares 22%-28% aa sequence identity with human B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD‑L2. Alternate splicing of human B7-H4 generates an additional isoform that lacks the first Ig-like domain. B7-H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow-derived mesenchymal stem cells
(4-8). Following binding to activated T cells, B7-H4 serves as a co‑inhibitor of the T cell response. This is accomplished by reverse signaling that can induce either cell cycle arrest, or apoptosis in B7-H4 expressing cells (2-5, 9, 10). B7‑H4 is up‑regulated in several carcinomas in correlation with tumor progression and metastasis (2, 7, 11, 12). A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, and is correlated with advanced disease status (13-15). Soluble B7‑H4 functions as a decoy molecule that blocks the inhibitory influence of B7‑H4 on immune activation (15). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (16).

References

  1. Yi, K.H. and L. Chen (2009) Immunol. Rev. 229:145.
  2. Guo, Y. and A.Y. Wang (2015) Front. Immunol. 6:421.
  3. Zang, X. et al. (2003) Proc. Natl. Acad. Sci. USA 100:10388.
  4. Prasad, V.R. et al. (2003) Immunity 18:863.
  5. Sica, G.L. et al. (2003) Immunity 18:849.
  6. Kryczek, I. et al. (2006) J. Exp. Med. 203:871.
  7. Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842.
  8. Xue, Q. et al. (2010) Stem Cells Dev. 19:27.
  9. Song, H. et al. (2008) Cancer Lett. 266:227.
  10. Park, G.B. et al. (2009) Immunology 128:360.
  11. Zang, X. et al. (2007) Proc. Natl. Acad. Sci. USA 104:19458.
  12. Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10391.
  13. Simon, I. et al. (2006) Cancer Res. 66:1570.
  14. Thompson, R.H. et al. (2008) Cancer Res. 68:6054.
  15. Azuma, T. et al. (2009) PloS Med. 6:e1000166.
  16. Suh, W.-K. et al. (2006) Mol. Cell. Biol. 26:6403.

Long Name

B7 Homolog 4

Alternate Names

B7H4, B7S1, B7x, Vtcn1

Entrez Gene IDs

79679 (Human); 242122 (Mouse); 295322 (Rat)

Gene Symbol

VTCN1

UniProt

Q7Z7D3

Additional B7-H4 Products

Product Documents for Human B7‑H4 PE‑conjugated Antibody

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Product Specific Notices for Human B7‑H4 PE‑conjugated Antibody

For research use only

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