Human B7-H7/HHLA2 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB80841T-025
Detection  of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and  eGFP by Flow Cytometry.
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Human B7-H7/HHLA2 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-H7/HHLA2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 907812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
Met1-Asn344
Accession # Q9UM44
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and  eGFP by Flow Cytometry. View Larger

Detection of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human B7-H7/HHLA2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human B7-H7/HHLA2 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB80841T). Quadrant markers were set based on control antibody staining (Catalog # IC002T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
Long Name
B7 Homolog 7
Entrez Gene IDs
11148 (Human); 102137704 (Cynomolgus Monkey)
Alternate Names
B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2

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